<!DOCTYPE html>
<html>
<body>
<audio src="horse.mp3" id="mp3" preload="auto"></audio>
<a href="javascript:play_single_sound();">Play 5-sec sound on single channel</a>
<script type="text/javascript">
function play_single_sound() {
document.getElementById('mp3').play();
}
</script>
</body>
</html>